ADHDgene Database

Hot Results

Quick Search

Keyword:
Type:

Large-scale studies

Data Summary

Published Variant
- SNP: 1391
- CNV: 398
- Others: 173
Published Gene: 359
Published Region: 128
Pathway by PBA: 8
Study: 361

Study Report

Basic Info

Reference	Wigg KG, 200616856124
Citation	Wigg K. G., Takhar A., Ickowicz A., Tannock R., Kennedy J. L., Pathare T., Malone M., Schachar R. and Barr C. L. (2006) "Gene for the serotonin transporter and ADHD: no association with two functional polymorphisms." Am J Med Genet B Neuropsychiatr Genet, 141B(6): 566-70.
Study Design	family-based
Study Type	Candidate-gene association study
Sample Size	209 small nuclear families with 249 affected children
Predominant Ethnicity	Caucasian
Population	Canada
Gender	81% male and 19% female
Age Group	Children/Adolescents : 7-16 years

Detail Info

Summary	They investigated the relationship of several variants to ADHD: the promoter polymorphisms, SNP in the 3' untranslated region (3'UTR) with a reported association to ADHD and a rare, non-synonymous coding SNP. These polymorphisms were genotyped in 209 ADHD families identified through an affected proband. They did not find evidence for an association of these polymorphisms, or haplotypes of these polymorphisms, to ADHD in this sample.
Total Sample	The study sample consisted of 209 small nuclear families with 249 affected children (probands and siblings) included in the analyses. Of the cases that were used in this study, 81% were male and19% were female.
Sample Collection	European Caucasian, African Canadian, Native Canadian, and families from the Caribbean
Diagnosis Description	Subjects participating in the study were required to meet the criteria for one of the three DSM-IV ADHD subtypes based on information from questionnaires, semistructured interviews for parents [parent interview for child symptoms (PICS)] [Ickowicz et al., 2002] and teachers (teacher telephone interview for children's academic performance, attention, behavior, and learning (TTI): DSM-IV Version) [Tannock et al., 2002], and child assessment as previously described [Barr et al., 2000a,b, 2001a,c; Misener et al., 2004]. For the DSM-IV diagnostic criteria, 62% of the children were of the combined subtype, 14% were of the hyperactive/impulsive subtype, and 24% were of the primarily inattentive subtype.
Technique	DNA was extracted directly from blood lymphocytes using a high salt extraction method [Miller et al., 1988]. The marker rs3813034 was genotyped with the ABI 7900-HT Sequence Detection System (Applied Biosystems, Foster City, CA) using the TaqMan 5' nuclease assay for allelic discrimination. The promoter polymorphism was amplified using the primer pairs 5'-GGCGTTGCCGCTCTGAAT-3' and 5'-GAGGGACTGAGCTGGACAACCCAC- 3'. The most frequently observed alleles yield either a 486 bp (short) or 529 bp (long) product. More detailed description about Isolation of DNA and Marker Genotyping could be found in the original publication.
Analysis Method	For the categorical analysis, the TDT statistic was calculated using the extended TDT (ETDT) program [Sham and Curtis, 1995]. The transmission of the haplotypes was analyzed with the TRANSMIT program [Clayton, 1999]. For the analysis of the quantitative measures, the FBAT programwas used [Horvath et al., 2001] with an additive model.
Result Description	They did not find evidence for an association of these polymorphisms, or haplotypes of these polymorphisms, to ADHD in this sample.

SNPs reported by this study (count: 1)

Other variant reported by this study (count: 1)

Variant Name	Allele Change	Risk Allele	Statistical Values	Author Comments	Result of Statistical Analysis
5HTTLPR	short/long		S_A TDT P-value=0.456, X²=0.556; L_...... S_A TDT P-value=0.456, X²=0.556; L_A TDT P-value=0.419, X²=0.654; L_G TDT P-value=0.893, X²=0.018; L_A+L_G P-value=0.519 More...	no evidence of biased transmission	Non-significant

Genes reported by this study (count: 1)