Study Report

Basic Info
Reference |
Li J, 2006(e)16701945
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Citation |
Li J., Wang Y., Zhou R., Wang B., Zhang H., Yang L. and Faraone S. V. (2006) "No association of attention-deficit/hyperactivity disorder with genes of the serotonergic pathway in Han Chinese subjects." Neurosci Lett, 403(1-2): 172-5.
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Study Design |
family-based |
Study Type |
Candidate-gene association study |
Sample Size |
HTR2A was successfully genotyped in a total of 311 trios. HTR5A was successfully genotyped in a total of 333 trios. HTR6 was successfully genotyped in a total of 298 trios. |
Predominant Ethnicity |
Mongoloid |
Population |
China |
Age Group |
Children/Adolescents
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Detail Info
Summary |
The current study examined five variants in three serotonin genes [those that code for serotonin receptors 2A (HTR2A), 5A (HTR5A) and 6 (HTR6)] in a relatively large sample of ADHD nuclear families. The transmission disequilibrium test (TDT) and the extended transmission disequilibrium test (ETDT) were performed to test for evidence of distorted transmission of alleles or haplotypes. No significant biased transmission was observed. These results do not support a substantial role of these serotonin gene in ADHD, however, additional work may be warranted before this association is definitively discounted. |
Total Sample |
Proband-parent ADHD trios were recruited from the child psychiatric clinics at Peking University Institute of Mental Health. HTR2A was successfully genotyped in a total of 311 trios. HTR5A was successfully genotyped in a total of 333 trios. HTR6 was successfully genotyped in a total of 298 trios. |
Sample Collection |
Han Chinese |
Diagnosis Description |
Proband-parent ADHD trios were recruited from the child psychiatric clinics at Peking University Institute of Mental Health. Affected individuals were diagnosed by at least two experienced clinicians according to the Diagnostic and Statistical Manual of Mental Disorders (DSM-IV) criteria for ADHD. Subjects were excluded if they showed evidence of bipolar affective disorder, childhood schizophrenia, autism, mental retardation or other neurological or complex medical illnesses. |
Technique |
Genomic DNA was extracted from blood using standard procedures. Both 102T>C and -1438A>G polymorphisms of the HTR2A gene and 267C>T of the HTR6 gene were genotyped according to protocols employed in previous studies. The same PCR reaction for -19G>C and 12A/T of the HTR5A gene was performed under the conditions described by Shimron- Abarbanell. Ten microlitres of PCR products were digested with BsaJI for more than 16 h, followed by BsrI for more than 16 h and then analyzed on polyacrylamide gel electrophoresis. Each gel was then immersed into water containing ethidium bromide for 30 min. Finally, products were analyzed with a Gel Doc 2000 imaging system (Bio-Rad, Hercules, CA) to detect and record the genotype of each sample. |
Analysis Method |
The transmission disequilibrium test (TDT) and the extended transmission disequilibrium test (ETDT) were performed to test for evidence of distorted transmission of alleles or haplotypes. |
Result Description |
No significant biased transmission was observed. These results do not support a substantial role of these serotonin gene in ADHD, however, additional work may be warranted before this association is definitively discounted. |

Other variant reported by this study (count: 5)
Variant Name |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result of Statistical Analysis |
HTR2A promoter 1438G/A |
G/A |
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TDT P-value=0.428, McNemar X2=0.628
TDT P-value=0.428, McNemar X2=0.628
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no biased transmission of any alleles was observed |
Non-significant
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HTR6 267C>T |
C/T |
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TDT P-value=0.954, McNemar X2=0.003
TDT P-value=0.954, McNemar X2=0.003
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no biased transmission of any alleles was observed |
Non-significant
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HTR5A 12A>T |
A/T |
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TDT P-value=0.626, McNemar X2=0.238
TDT P-value=0.626, McNemar X2=0.238
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no biased transmission of any alleles was observed |
Non-significant
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HTR5A -19G>C |
G/C |
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TDT P-value=0.528, McNemar X2=0.399
TDT P-value=0.528, McNemar X2=0.399
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no biased transmission of any alleles was observed |
Non-significant
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HTR2A T102C |
C/T |
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TDT P-value=0.428, McNemar X2=0.628
TDT P-value=0.428, McNemar X2=0.628
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no biased transmission of any alleles was observed |
Non-significant
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Genes reported by this study (count: 3)
Gene |
Statistical Values/Author Comments |
Result of Statistical Analysis |
HTR6 |
no biased transmission of any alleles of 267C>T was observed......
no biased transmission of any alleles of 267C>T was observed
More...
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Non-significant
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HTR5A |
biased transmission of either alleles or specific haplotypes......
biased transmission of either alleles or specific haplotypes was not observed to probands
More...
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Non-significant
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HTR2A |
no biased transmission of any alleles of the 102T>C and -143......
no biased transmission of any alleles of the 102T>C and -1438A>G was observed
More...
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Non-significant
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