Study Report

Basic Info
Reference |
Kent L, 2001(b)11409698
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Citation |
Kent L., Middle F., Hawi Z., Fitzgerald M., Gill M., Feehan C. and Craddock N. (2001) "Nicotinic acetylcholine receptor alpha4 subunit gene polymorphism and attention deficit hyperactivity disorder." Psychiatr Genet, 11(1): 37-40.
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Study Design |
family-based |
Study Type |
Candidate-gene association study |
Sample Size |
70 trios |
Predominant Ethnicity |
Caucasian |
Population |
United Kingdom, Ireland |
Gender |
61 (87%) male, 9 (13%) female |
Age Group |
Children/Adolescents
:
5-16 years (mean age=11.1 years, SD=3.0.)
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Detail Info
Summary |
The current study examined the alpha 4 receptor, one of the sites of action of ABT-418. A known Cfol polymorphism within the nicotinic acetylcholine alpha 4 receptor gene, CHRNA4, was studied in 70 ADHD parent-proband trios from an ongoing sample collection of children aged 6-12 with ADHD, according to DSM-IV criteria. Children with known major medical or psychiatric conditions or mental retardation (IQ < 70) were excluded from the study. The Transmission Disequilibrium Test demonstrated no evidence that variation at the nicotinic acetylcholine alpha 4 receptor Cfol polymorphism influences susceptibility to ADHD (P > 0.35). The continuing sample collection will enable further study of other potential nicotinic system polymorphisms in ADHD in more powerful samples. |
Total Sample |
The sample was recruited from several child psychiatry clinics in the United Kingdom and Ireland, and consisted of 70 parent-proband trios who were interviewed employing the Child and Adolescent Psychiatric Assessment (Angold et al., 1995). Probands were Caucasian, born in the United Kingdom or Ireland and were aged 5-16 years (mean age= 11.1 years, sd=3.0). |
Sample Collection |
The sample was recruited from several child psychiatry clinics in the United Kingdom and Ireland, and consisted of 70 parent-proband trios who were interviewed employing the Child and Adolescent Psychiatric Assessment (Angold et al., 1995). |
Diagnosis Description |
All probands fulfilled DSM-IV (USAn Psychiatric Association, 1994) diagnostic criteria for ADHD. Of the 70 probands, 59 (84%) were combined type, five (7%) were inatttentive subtype and six (9%) were hyper- active/impulsive subtype. |
Technique |
High molecular weight genomic DNA was extracted from either whole blood or cheekswab according to routine procedures. A same-sense T->C exchange in exon 5 of the CHRNA4 gene (bp 1545) creates a Cfo1 digestion site. This polymorphism was typed by PCR using a previously described method (Steinlein et al., 1997). |
Analysis Method |
Genotype frequencies in parents were compared with frequencies expected under Hardy-Weinberg equilibrium using the X2 goodness of fit test. The Transmission Disequilibrium Test (TDT) (Spielman et al., 1993) was employed to test for allelic association. |
Result Description |
The Transmission Disequilibrium Test demonstrated no evidence that variation at the nicotinic acetylcholine alpha 4 receptor Cfol polymorphism influences susceptibility to ADHD (P > 0.35). The continuing sample collection will enable further study of other potential nicotinic system polymorphisms in ADHD in more powerful samples. |

Other variant reported by this study (count: 1)
Variant Name |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result of Statistical Analysis |
CHRNA4 exon5 T/C CfoI |
T/C |
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allelic TDT P-value=0.35, McNemar X2=0.89
allelic TDT P-value=0.35, McNemar X2=0.89
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TDT analysis demonstrated no allelic association |
Non-significant
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Genes reported by this study (count: 1)
Gene |
Statistical Values/Author Comments |
Result of Statistical Analysis |
CHRNA4 |
this study did not support an association between the nicoti......
this study did not support an association between the nicotinic acetylcholine alpha 4 receptor Cfo1 polymorphism and ADHD
More...
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Non-significant
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