ADHDgene Database

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Large-scale studies

Data Summary

Published Variant
- SNP: 1391
- CNV: 398
- Others: 173
Published Gene: 359
Published Region: 128
Pathway by PBA: 8
Study: 361

Study Report

Basic Info

Reference	Li J, 2006(d)17099886
Citation	Li J., Zhang X., Wang Y., Zhou R., Zhang H., Yang L., Wang B. and Faraone S. V. (2006) "The serotonin 5-HT1D receptor gene and attention-deficit hyperactivity disorder in Chinese Han subjects." Am J Med Genet B Neuropsychiatr Genet, 141B(8): 874-6.
Study Design	family-based
Study Type	Candidate-gene association study
Sample Size	272 trios
Predominant Ethnicity	Mongoloid
Population	China
Gender	236 were male and 36 were female
Age Group	Children/Adolescents : mean age10.5,SD=2.5 years

Detail Info

Summary	The current study genotyped two variants of HTR1D gene in 272 ADHD trios of Chinese ethnicity, that is 1350T>C in the coding region and 1236A>G in 3'-UTR by the use of transmission disequilibrium test (TDT). The A allele of the 1236A>G polymorphism exhibited both a trend toward preferential transmission to ADHD probands and a significant preferential transmission to probands of ADHDC. Additional polymorphisms in this gene need to be studied further.
Total Sample	Two hundred seventy-two nuclear families participated in the present study. ADHD probands were recruited from the ADHD outpatient clinic at the Child and Adolescent Psychiatric Division of the Sixth Hospital, Peking University in Beijing, PRC.
Sample Collection	Han Chinese
Diagnosis Description	All probands were interviewed by at least two different child psychiatrists with the aid of their biological parents and teacher and fulfilled DSM-IV diagnostic criteria for ADHD [USAn Psychiatric Association, 1994]. One hundred nine (40.0%) probands met criteria for ADHD combined type (ADHD-C), 17 (6.3%) met criteria for ADHD hyperactive-impulsive type (ADHD-HI), and 146 (53.7%) met criteria for ADHD inattentive type (ADHD-I).
Technique	Genomic DNA was extracted from whole blood by standard procedures. 1350T>C polymorphism was genotyped using the conditions described by Ozaki et al. [1995]. 1236A>G polymorphism was amplified with forward primer 5'-TCC TGT AAC CTC ATC GGG ATT G-3' and backward primer 5'-CGA GAG GGC AAG ACT CCG TC-3'. After initial denaturation at 95^oC for 4 min, 35 cycles were carried out at 96^oC for 30 sec, 53^oC for 30 sec, and 72^oC for 30 sec, followed by a final step of elongation at 72^oC for 7 min. PCR products (10 ml) were digested with five units of EcoRV (MBI Fermentas, Vilnius, Lithuania) in 16 ml reaction volumes for 12 hr at 37^oC. The digested PCR products were run on a 2.5% agarose gel at 100 V for 1.5 hr, then the agarose gel into water containing ethidium bromide for half an hour. Finally, they were analyzed with Gel Doc 2000 imaging system to detect and record the genotype of each sample. After digestion, the A variant was cut into two fragments of 66 and 170 bp, while the G variant was uncut and was 236 bp. Genotypes were read by two persons, both of whom were blind to the identity of the samples.
Analysis Method	The transmission disequilibrium test (TDT) [Spielman et al., 1993] was performed to test for linkage and association of the two polymorphisms with ADHD.
Result Description	The A allele of the 1236A>G polymorphism exhibited both a trend toward preferential transmission to ADHD probands (X²=3.815, P-value=0.051) and a significant preferential transmission to probands of ADHDC (X²=4.198, P-value=0.040).

Other variant reported by this study (count: 2)

Variant Name	Allele Change	Risk Allele	Statistical Values	Author Comments	Result of Statistical Analysis
HTR1D 1350T>C	C/T		TDT P-value=0.275, McNemar X²=1.19 for ADHD pheno...... TDT P-value=0.275, McNemar X²=1.19 for ADHD phenotype; TDT P-value=0.185, McNemar X²=1.754 for ADHD-C phenotype; TDT P-value=0.907, McNemar X²=0.014 for ADHD-I phenotype More...	no biased transmission was observed	Non-significant
HTR1D 3'-UTR 1236A>G	A/G		TDT P-value=0.051, McNemar X²=3.815 for ADHD phen...... TDT P-value=0.051, McNemar X²=3.815 for ADHD phenotype; TDT P-value=0.04, McNemar X²=4.198 for ADHD-C phenotype; TDT P-value=0.425, McNemar X²=0.637 for ADHD-I phenotype More...	a trend toward preferential transmission to ADHD probands and a significant preferential transmission to probands of ADHDC	Significant

Genes reported by this study (count: 1)