ADHDgene Database

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Large-scale studies

Data Summary

Published Variant
- SNP: 1391
- CNV: 398
- Others: 173
Published Gene: 359
Published Region: 128
Pathway by PBA: 8
Study: 361

Study Report

Basic Info

Reference	Payton A, 200312960752
Citation	Payton A., Turic D., Langley K., Mills S., Lawson D. C., Van den Bree M., Owen M. J., O'Donovan M. C., Ollier W., Worthington J. and Thapar A. (2003) "No evidence of association between HLA-DRB1 and attention deficit hyperactivity disorder." Psychiatr Genet, 13(3): 183-5.
Study Design	case-control and family-based
Study Type	Candidate-gene association study
Sample Size	173 probands and their parents (including 121 trios) and 367 controls
Predominant Ethnicity	Caucasian
Population	United Kingdom
Gender	154 boys and 19 girls in the family-based sample
Age Group	Children/Adolescents : 6-16 years

Detail Info

Summary	There has been a recent resurgence in interest in the role of autoimmunity in childhood neuropsychiatric disorders. Significant association between HLA-DRB1 and attention deficit hyperactivity disorder (ADHD) in a case-control study of 31 subjects has been reported but there have been no other published studies following up these results. They attempted to replicate these findings. In a well-characterized sample of 173 children with ADHD, using a fully automated sequence-specific oligonucleotide method for HLA genotyping, association between ADHD and HLA-DRB1 was tested for using the Transmission Disequilibrium Test and case-control analysis. Transmission Disequilibrium Test analysis yielded a chi-square of 10.694 with a simulated global P value of 0.1641 for the full sample, and a chi-square value of 11.307 with a simulated global P value of 0.1323 for the complete trios only.
Total Sample	The sample comprised 173 children who fulfilled either ICD-10 or DSM-IV/DSM-III-R criteria for ADHD, of whom 154 were boys and 19 were girls. Venous blood samples were obtained from 173 probands, of which 121 were from complete child and parent trios. The control group (n=367) was comprised of British Caucasian healthy blood donors and healthy individuals selected at random from general practice registers in East Anglia, United Kingdom.
Sample Collection	Families with children who had been referred with a suspected diagnosis of ADHD were recruited from Child and Adolescent Psychiatry and Paediatric Clinics in Cardiff, South Wales, Avon, Greater Manchester and Cheshire, United Kingdom. The control group (n=367) was comprised of British Caucasian healthy blood donors and healthy individuals selected at random from general practice registers in East Anglia, United Kingdom.
Diagnosis Description	Mothers were interviewed about their children, using the Child and Adolescent Psychiatric Assessment (Angold et al., 1995), a research diagnostic interview. Reports of ADHD symptoms and impairment at school were also obtained using a semi-structured teacher telephone interview (Child ADHD Teacher Telephone Interview; Holmes et al., 2000). Diagnoses were assigned according to ICD-10, DSM-IV and DSM-III-R criteria. Characteristics of the sample and assessment methods have been described in greater detail previously (Holmes et al., 2000).
Technique	Exon 2 PCR amplification of the DRB1 gene was performed using the INNO-LiPA key amplification reagents in a final reaction volume of 20 ul containing 0.2U Taq (BioTaq, Bioline, London, United Kingdom) and 50 ng DNA. Families and controls were genotyped using the INNO-LiPA reverse hybridization (Buyse et al., 1993) typing system.
Analysis Method	Transmission Disequilibrium Test (TDT) analysis was undertaken for 167 complete parent-child trios and mother-child duos using the statistical package TRANSMIT (Clayton, 1999). Case-control analysis was also undertaken for the total sample of 173 probands, using an in-house statistical package (JWOOLF) to allow for comparison with the findings reported by Odell et al. (1997).
Result Description	Transmission Disequilibrium Test analysis yielded a chi-square of 10.694 with a simulated global P value of 0.1641 for the full sample, and a chi-square value of 11.307 with a simulated global P value of 0.1323 for the complete trios only.

Other variant reported by this study (count: 1)

Variant Name	Allele Change	Risk Allele	Statistical Values	Author Comments	Result of Statistical Analysis
HLA-DRB1 alleles01-10	allele 1-10		allelic TDT simulated global P-value=0.16, X²=10....... allelic TDT simulated global P-value=0.16, X²=10.7 for the full sample, allelic TDT simulated global P-value=0.13, X²=11.31 for the complete trios; allelic TDT simulated global P-value=0.86, X²=0.03 for DRB01, allelic TDT simulated global P-value=0.25, X²=1.22 for DRB04 in the full sample; OR=1.1 for DRB01, OR=1.0 for DRB04 in the case-control analysis More...	no preferential transmission of any allele was noted and the case-control findings were also negative	Non-significant

Genes reported by this study (count: 1)